Application
Immunofluorescence of OGFOD1 in Daudi cells with OGFOD1 antibody at 20 μg/mL.Application
Western blot analysis of OGFOD1 in Daudi cell lysate with OGFOD1 antibody at 1 μg/mL.| Product Name | OGFOD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | OGFOD1 Antibody: OGFOD1, a member of the 2-OG-Fe(II) dioxygenase family, plays an important role in ischemic survival and stress. It is an oxidoreductase containing a Prolyl 4-hydroxylase alpha subunit. An OGFOD1 iron binding residue is required for ATPAF1 gene expression. As a novel stress granule component, OGFOD1 plays important proapoptotic roles in the regulation of translation and HRI-mediated phosphorylation of eIF2alpha in cells.1) Saito K, Adachi N, Koyama H et al. OGFOD1, a member of the 2-oxoglutarate and iron dependent dioxygenase family, functions in ischemic signaling. FEBS Lett. 2010; 584:3340-7. |
| Immunogen | OGFOD1 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human OGFOD1. The immunogen is located within the first 50 amino acids of OGFOD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| OGFOD1 antibody can be used for detection of OGFOD1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OGFOD1 |
|---|---|
| Alternative Names | OGFOD1 Antibody: TPA1 KIAA1612 TPA1 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1 Termination and polyadenylation 1 homolog |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | OGFOD1 antibody is predicted to not cross-react with other TPA1 protein family members. At least two isoforms are known to exist; this antibody will only detect the larger isform. |
Application
Immunofluorescence of OGFOD1 in Daudi cells with OGFOD1 antibody at 20 μg/mL.
Application
Western blot analysis of OGFOD1 in Daudi cell lysate with OGFOD1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
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| Application Notes | OGFOD1 antibody can be used for detection of OGFOD1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
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| Storage Instructions | OGFOD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | OGFOD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
