Application
Western blot analysis of OGT antibody in mouse bladder tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with OGT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | OGT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal.1) Roeder,R.G., Nature 459 (7245), 455-459 (2009) |
| Immunogen | This OGT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1017-1046 amino acids from the C-terminal region of human OGT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | OGT |
|---|---|
| Alternative Names | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit O-GlcNAc transferase subunit p110 O-linked N-acetylglucosamine transferase 110 kDa subunit OGT OGT |
| Molecular Weight(MW) | 117 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Western blot analysis of OGT antibody in mouse bladder tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with OGT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
