Application
Immunofluorescence of OLIG1 in human brain tissue with OLIG1 antibody at 20 μg/mL.Application
Immunohistochemistry of OLIG1 in human brain tissue with OLIG1 antibody at 5 μg/mL.Application
Western blot analysis of OLIG1 in A549 cell lysate with OLIG1 antibody at 1 μg/ml.| Product Name | OLIG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OLIG1 Antibody: BHLHB6, BHLHE21, BHLHB6, Oligodendrocyte transcription factor 1, Class B basic helix-loop-helix protein 6, Oligo1 |
| Product description | The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1). Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2). OLIG1 is first expressed in the dorsal portion of the p3 progenitor domain of the ventral neural tube while OLIG2 is first observed in the ventral most p3 domain (2). OLIG1 has been shown to be a SMAD cofactor involved in cell motility induced by transforming growth factor-beta (TGF-beta) (3).1) Zhou Q, Wang S, and Anderson DJ. Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors. Neuron 2000; 25:331-43. |
| Immunogen | OLIG1 antibody was raised against an 18 amino acid peptide near the amino terminus of human OLIG1. The immunogen is located within amino acids 50 - 100 of OLIG1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| OLIG1 antibody can be used for detection of OLIG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OLIG1 |
|---|---|
| Alternative Names | OLIG1 Antibody: BHLHB6 BHLHE21 BHLHB6 Oligodendrocyte transcription factor 1 Class B basic helix-loop-helix protein 6 Oligo1 |
| Molecular Weight(MW) | Predicted: 30 kDa Observed: 29 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | OLIG1 antibody is human specific. It is predicted to not cross-react with other members of the OLIG family of proteins. |
Application
Immunofluorescence of OLIG1 in human brain tissue with OLIG1 antibody at 20 μg/mL.
Application
Immunohistochemistry of OLIG1 in human brain tissue with OLIG1 antibody at 5 μg/mL.
Application
Western blot analysis of OLIG1 in A549 cell lysate with OLIG1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | OLIG1 antibody can be used for detection of OLIG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | OLIG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | OLIG1 Antibody is supplied in PBS containing 0.02% sodium azide. |
