Application
Immunohistochemistry of OMI in human liver tissue with OMI antibody at 2 μg/mL.Application
Western blot analysis of OMI in U937 lysate with Omi antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.Application
Immunofluorescence of OMI in Human Liver cells with OMI antibody at 20 μg/mL.| Product Name | OMI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OMI Antibody: OMI, PARK13, PRSS25, OMI, Serine protease HTRA2, mitochondrial, High temperature requirement protein A2, HtrA2 |
| Product description | OMI Antibody: Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication. Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases. The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs. Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs. Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis.1) Crook NE, Clem RJ, and Miller LK. An apoptosis inhibiting baculovirus gene with a zinc finger like motif. J. Virol. 1993; 67:2168-2174. |
| Immunogen | OMI antibody was raised against a peptide corresponding to 16 amino acids near the C-terminus of human Omi. The immunogen is located within amino acids 70 - 120 of OMI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| OMI antibody can be used for detection of OMI by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HTRA2 |
|---|---|
| Alternative Names | OMI Antibody: OMI PARK13 PRSS25 OMI Serine protease HTRA2 mitochondrial High temperature requirement protein A2 HtrA2 |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of OMI in human liver tissue with OMI antibody at 2 μg/mL.
Application
Western blot analysis of OMI in U937 lysate with Omi antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Application
Immunofluorescence of OMI in Human Liver cells with OMI antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1215 - U937 Cell Lysate |
|---|---|
| Application Notes | OMI antibody can be used for detection of OMI by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | OMI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | OMI Antibody is supplied in PBS containing 0.02% sodium azide. |
