Application
Immunohistochemistry of OMI in human colon tissue with OMI antibody at 10 μg/mL.Application
Immunofluorescence of OMI in human colon tissue with OMI antibody at 20 μg/mL.Application
Western blot analysis of OMI in human colon cell lysates with OMI antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | OMI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OMI Antibody: OMI, PARK13, PRSS25, OMI, Serine protease HTRA2, mitochondrial, High temperature requirement protein A2, HtrA2 |
| Product description | OMI Antibody: Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication. Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases. The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs. Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs. Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis.1) Crook NE, Clem RJ, and Miller LK. An apoptosis inhibiting baculovirus gene with a zinc finger like motif. J. Virol. 1993; 67:2168-2174. |
| Immunogen | OMI antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human OMI. The immunogen is located within amino acids 120 - 170 of OMI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| OMI antibody can be used for detection of OMI by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HTRA2 |
|---|---|
| Alternative Names | OMI Antibody: OMI PARK13 PRSS25 OMI Serine protease HTRA2 mitochondrial High temperature requirement protein A2 HtrA2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (85%), Bovine: (90%) |
Application
Immunohistochemistry of OMI in human colon tissue with OMI antibody at 10 μg/mL.
Application
Immunofluorescence of OMI in human colon tissue with OMI antibody at 20 μg/mL.
Application
Western blot analysis of OMI in human colon cell lysates with OMI antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | OMI antibody can be used for detection of OMI by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | OMI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | OMI Antibody is supplied in PBS containing 0.02% sodium azide. |
