Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in IHC on Human Brain, cerebellum at 5.0 ug/ml.| Product Name | ONECUT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ONECUT1, HNF-6, HNF6, HNF6A |
| Product description | ONECUT1 belongs to the CUT homeobox family. It contains 1 CUT DNA-binding domain and 1 homeobox DNA-binding domain. It is a transcriptional activator. ONECUT1 binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. It is important for liver genes transcription.1) Jiang, X., (2008) Oncol. Rep. 19 (1), 157-163. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ONECUT1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ONECUT1 antibody can be used for detection of ONECUT1 by ELISA at 1:312500. ONECUT1 antibody can be used for detection of ONECUT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ONECUT1 |
|---|---|
| Alternative Names | ONECUT1 HNF-6 HNF6 HNF6A |
| Molecular Weight(MW) | 51 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Brain, cerebellum at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ONECUT1 antibody can be used for detection of ONECUT1 by ELISA at 1:312500. ONECUT1 antibody can be used for detection of ONECUT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ONECUT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
