Application
HEK293T cells transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF6 (RC218219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF6 .Application
Anti-HNF6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HNF6Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HNF6 mouse monoclonal antibody.| Product Name | HNF6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [ 4F12] to HNF6Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 85-198 of human HNF6 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4F12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| IHC 1:50 Antigen Retrieval: 0.01M Sodium Citrate Buffer, pH 6.0 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ONECUT1 HNF6 HNF6A HNF-6 HNF 6 |
|---|---|
| Target | HNF6 |
| Tissue Specificity | is specific for amino acids 85-198 of human HNF6 |
Application
HEK293T cells transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF6 (RC218219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF6 .
Application
Anti-HNF6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HNF6
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HNF6 mouse monoclonal antibody.| Application Notes | IHC 1:50 Antigen Retrieval: 0.01M Sodium Citrate Buffer, pH 6.0 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
