Application
Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 μg/ml.Application
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 μg/mL.Application
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.Application
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 μg/mL.| Product Name | ORAI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ORAI1 Antibody: IMD9, ORAT1, CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1 |
| Product description | ORAI1 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.1) Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521. |
| Immunogen | ORAI1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human ORAI1. The immunogen is located within the first 50 amino acids of ORAI1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ORAI1 |
|---|---|
| Alternative Names | ORAI1 Antibody: IMD9 ORAT1 CRACM1 TMEM142A Calcium release-activated calcium channel protein 1 Protein orai-1 |
| Molecular Weight(MW) | Predicted: 33 kDa Observed: 50 kDa |
| Sequence Similarities | 4) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3. |
Application
Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 μg/ml.
Application
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 μg/mL.
Application
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.
Application
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1316 - Human Ovary Tissue Lysate |
|---|---|
| Application Notes | ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ORAI1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ORAI1 Antibody is supplied in PBS containing 0.02% sodium azide. |
