Application
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 5 μg/mL.Application
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 μg/mL.Application
Immunofluorescence of ORAI1 in Human Spleen tissue with ORAI1 antibody at 20 ug/mL.Application
Western blot analysis of ORAI1 in human spleen tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | ORAI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ORAI1 Antibody [6D11A11] : IMD9, ORAT1, CRACM1, TMEM142A |
| Product description | ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.1) Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521. |
| Immunogen | Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ELISAIFIHC-PWB |
| ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ORAI1 |
|---|---|
| Alternative Names | ORAI1 Antibody [6D11A11] : IMD9 ORAT1 CRACM1 TMEM142A |
| Molecular Weight(MW) | Predicted: 33 kDa Observed: 50 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | ORAI1 antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3. |
Application
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 5 μg/mL.
Application
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of ORAI1 in Human Spleen tissue with ORAI1 antibody at 20 ug/mL.
Application
Western blot analysis of ORAI1 in human spleen tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1366 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ORAI1 monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
