Application
Immunocytochemistry of ORAI2 in Jurkat cells with ORAI2 antibody at 5 μg/mL.Application
Immunofluorescence of ORAI2 in Jurkat cells with ORAI2 antibody at 20 μg/mL.Application
Western blot analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Product Name | ORAI2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ORAI2 Antibody: CBCIP2, C7orf19, MEM142B, TMEM142B, CBCIP2, PP1729, Protein orai-2, CAP-binding protein complex-interacting protein 2 |
| Product description | ORAI2 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1.1) Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521. |
| Immunogen | ORAI2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ORAI2. The immunogen is located within the last 50 amino acids of ORAI2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ORAI2 antibody can be used for detection of ORAI2 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ORAI2 |
|---|---|
| Alternative Names | ORAI2 Antibody: CBCIP2 C7orf19 MEM142B TMEM142B CBCIP2 PP1729 Protein orai-2 CAP-binding protein complex-interacting protein 2 |
| Molecular Weight(MW) | Predicted: 19, 28 kDa Observed: 29 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | At least two isoforms of ORAI2 are known to exist; this antibody will detect both isoforms. ORAI antibody is predicted not to cross-react with ORAI1 or ORAI3. |
Application
Immunocytochemistry of ORAI2 in Jurkat cells with ORAI2 antibody at 5 μg/mL.
Application
Immunofluorescence of ORAI2 in Jurkat cells with ORAI2 antibody at 20 μg/mL.
Application
Western blot analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ORAI2 antibody can be used for detection of ORAI2 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ORAI2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ORAI2 Antibody is supplied in PBS containing 0.02% sodium azide. |
