ORAI2 is a multi-pass membrane protein, and it belongs to the Orai family. It is a Ca (2+) release-activated Ca (2+)-like (CRAC-like) channel subunit which mediates Ca (2+) influx and increase in Ca (2+)-selective current by synergy with the Ca (2+) sensor, STIM1.1) DeHaven, W.I., (2007) J. Biol. Chem. 282 (24), 17548-17556.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ORAI2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ORAI2 antibody can be used for detection of ORAI2 by ELISA at 1:1562500. ORAI2 antibody can be used for detection of ORAI2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1219 - MCF7 Cell Lysate
Application Notes
ORAI2 antibody can be used for detection of ORAI2 by ELISA at 1:1562500. ORAI2 antibody can be used for detection of ORAI2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ORAI2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.