Application
Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 5 μg/mL.Application
Immunofluorescence of ORAI3 in A20 cells with ORAI3 antibody at 10 μg/mL.Application
Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4 μg/mL.| Product Name | ORAI3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ORAI3 Antibody: TMEM142C, TMEM142C, Protein orai-3, Transmembrane protein 142C |
| Product description | ORAI3 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.1) Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521. |
| Immunogen | ORAI3 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3. The immunogen is located within amino acids 200 - 250 of ORAI3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ORAI3 antibody can be used for detection of ORAI3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ORAI3 |
|---|---|
| Alternative Names | ORAI3 Antibody: TMEM142C TMEM142C Protein orai-3 Transmembrane protein 142C |
| Molecular Weight(MW) | Predicted: 32 kDa Observed: 34 kDa |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
| Tissue Specificity | ORAI3 antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. |
Application
Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 5 μg/mL.
Application
Immunofluorescence of ORAI3 in A20 cells with ORAI3 antibody at 10 μg/mL.
Application
Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | ORAI3 antibody can be used for detection of ORAI3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ORAI3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ORAI3 Antibody is supplied in PBS containing 0.02% sodium azide. |
