Application
Immunofluorescence of ORAI3 in human spleen tissue with ORAI3 antibody at 5 μg/ml.Application
Immunohistochemistry of ORAI3 in rat spleen tissue with ORAI3 antibody at 2.5 μg/mL.Application
Immunofluorescence of ORAI3 in rat spleen tissue with ORAI3 antibody at 5 μg/mL.Application
Western blot analysis of ORAI3 in rat spleen lysate with ORAI3 antibody at 1 μg/mL.| Product Name | ORAI3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ORAI3 Antibody [2H2G9] : TMEM142C |
| Product description | ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.1) Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521. |
| Immunogen | A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAIFIHC-PWB |
| ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ORAI3 |
|---|---|
| Alternative Names | ORAI3 Antibody [2H2G9] : TMEM142C |
| Molecular Weight(MW) | Predicted: 32 kDa Observed: 32 kDa |
Application
Immunofluorescence of ORAI3 in human spleen tissue with ORAI3 antibody at 5 μg/ml.
Application
Immunohistochemistry of ORAI3 in rat spleen tissue with ORAI3 antibody at 2.5 μg/mL.
Application
Immunofluorescence of ORAI3 in rat spleen tissue with ORAI3 antibody at 5 μg/mL.
Application
Western blot analysis of ORAI3 in rat spleen lysate with ORAI3 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1466 - Rat Spleen Tissue Lysate |
|---|---|
| Application Notes | ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ORAI3 monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
