Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.Application
Antibody used in WB on HepG2 at 1.25 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Product Name | OTC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OTC, OCTD |
| Product description | OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.1) Tanaka, A., (2005) J. Gastroenterol. 40 (1), 106-107. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human OTC. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| OTC antibody can be used for detection of OTC by ELISA at 1:312500. OTC antibody can be used for detection of OTC by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | OTC |
|---|---|
| Alternative Names | OTC OCTD |
| Molecular Weight(MW) | 39 kDa |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Application
Antibody used in WB on HepG2 at 1.25 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | OTC antibody can be used for detection of OTC by ELISA at 1:312500. OTC antibody can be used for detection of OTC by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store OTC antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
