Application
Flow cytometric analysis of ATDC5 cells using OTC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatoarcinoma with OTC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in SW620 cell line and mouse liver，rat liver lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.| Product Name | OTC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ornithine carbamoyltransferase, mitochondrial, Ornithine transcarbamylase, OTCase, OTC |
| Product description | OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia.1) Hansmannel,F., et.al., Neurosci. Lett. 449 (1), 76-80 (2009) |
| Immunogen | This OTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-98 amino acids from the Central region of human OTC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OTC |
|---|---|
| Alternative Names | Ornithine carbamoyltransferase mitochondrial Ornithine transcarbamylase OTCase OTC |
| Molecular Weight(MW) | 40 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of ATDC5 cells using OTC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatoarcinoma with OTC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in SW620 cell line and mouse liver，rat liver lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
