Application
OTU7B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).| Product Name | OTU7B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OTU domain-containing protein 7B, Cellular zinc finger anti-NF-kappa-B protein, Zinc finger A20 domain-containing protein 1, Zinc finger protein Cezanne, OTUD7B, ZA20D1 |
| Product description | OTU7B has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. Hydrolyzes both linear and branched forms of polyubiquitin. Negative regulator of nuclear factor NF-kappa-B.1) Bremm, A., et al. Nat. Struct. Mol. Biol. 17(8):939-947(2010) |
| Immunogen | This OTU7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-784 amino acids from the C-terminal region of human OTU7B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OTUD7B |
|---|---|
| Alternative Names | OTU domain-containing protein 7B Cellular zinc finger anti-NF-kappa-B protein Zinc finger A20 domain-containing protein 1 Zinc finger protein Cezanne OTUD7B ZA20D1 |
| Molecular Weight(MW) | 93 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
OTU7B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
