WB
Jurkat cells were subjected to SDS PAGE followed by western blot with OTUD7B antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffinembedded human kidney using OTUD7B antibody at dilution of 1:50 (under 40x lens).IP
IP result of anti-OTUD7B (IP:4ug; Detection:1:800) with Jurkat cells lysate 2500ug.| Product Name | OTUD7B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | OTUD7B fusion protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:1000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 550ug/ml |
| Purification | Affinity purified |
| Gene Symbol | OTUD7B |
|---|---|
| Gene Synonyms | ZA20D1 CEZANNE |
| Gene Full Name | OTU deubiquitinase 7B |
| Gene Summary | Enables Lys48-specific deubiquitinase activity and thiol-dependent deubiquitinase. Involved in several processes, including negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of macromolecule metabolic process; and protein deubiquitination. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | CEZANNE OTU domain containing 7B OTU7B OTUD7B ZA20D1 Zinc finger protein Cezanne |
| Molecular Weight(MW) | 93kDa(Observed Molecular Weight 100kDa) |
WB
Jurkat cells were subjected to SDS PAGE followed by western blot with OTUD7B antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffinembedded human kidney using OTUD7B antibody at dilution of 1:50 (under 40x lens).
IP
IP result of anti-OTUD7B (IP:4ug; Detection:1:800) with Jurkat cells lysate 2500ug.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
