Application
OTU7B Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the OTU7B antibody detected the OTU7B protein (arrow).Application
OTU7B Antibody (C-term) (AP14281b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTU7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | OTU7B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OTUD7B; ZA20D1; OTU domain-containing protein 7B; Cellular zinc finger anti-NF-kappa-B protein; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZA20D1 |
|---|---|
| Alternative Names | OTUD7B ZA20D1 OTU domain-containing protein 7B Cellular zinc finger anti-NF-kappa-B protein Zinc finger A20 domain-containing protein 1 Zinc finger protein Cezanne |
| Molecular Weight(MW) | 92526 Da |
| Function | Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF- kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections. Mediates deubiquitination of EGFR. Has deubiquitinating activity toward 'Lys-11', 'Lys-48' or 'Lys-63'- linked polyubiquitin chains. In vitro, has preference for 'Lys- 11'-linked polyubiquitin chains; however such data are unsure in vivo. Hydrolyzes both linear and branched forms of polyubiquitin |
| Tissue Specificity | Widely expressed. Abundant in kidney, heart and fetal liver. Expressed differentially among B-cells at distinct developmental stages. Higher expression seen in primary immature B-cells as compared to the mature cells |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles be cytoplasm and the nucleus in a XPO1/CRM1-dependent manner |
| Entrez Gene | 56957 |
|---|---|
| Nucleotide Accession | NP_064590.2 |
Application
OTU7B Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the OTU7B antibody detected the OTU7B protein (arrow).
Application
OTU7B Antibody (C-term) (AP14281b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTU7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
