Application
Immunofluorescence of OTUD5 in Human Kidney cells with OTUD5 antibody at 20 μg/mL.Application
Immunohistochemistry of OTUD5 in mouse kidney tissue with OTUD5 antibody at 2.5 μg/mL.Application
Western blot analysis of OTUD5 in human kidney lysate with OTUD5 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | OTUD5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OTUD5 Antibody: DUBA, OTU domain-containing protein 5, Deubiquitinating enzyme A, DUBA |
| Product description | OTUD5 Antibody: OTUD5 is a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and OTUD5 has been shown to suppress the type I interferon (IFN-I)-dependent innate immune response by cleaving the polyubiquitin chain from TRAF3, an essential type I interferon adaptor protein. Cleavage results in disassociation of TRAF3 from a downstream signaling complex containing TBK1 and the disruption of the IFN-I signaling cascade, indicating that OTUD5 acts as a negative regulator of innate immune responses. It has been suggested that by suppressing IFN-I production, OTUD5 may function to inhibit the emergence of certain autoimmune disorders such as systemic lupus erythematosus. Multiple isoforms of OTUD5 are known to exist.1) Kayagi N, Phung Q, Chan S, et al. DUBA: A deubiquitinase that regultes type I interferon production. Science2007; 318:1628-32. |
| Immunogen | OTUD5 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human OTUD5. The immunogen is located within amino acids 400 - 450 of OTUD5. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PIPWB |
| OTUD5 antibody can be used for detection of OTUD5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OTUD5 |
|---|---|
| Alternative Names | OTUD5 Antibody: DUBA OTU domain-containing protein 5 Deubiquitinating enzyme A DUBA |
Application
Immunofluorescence of OTUD5 in Human Kidney cells with OTUD5 antibody at 20 μg/mL.
Application
Immunohistochemistry of OTUD5 in mouse kidney tissue with OTUD5 antibody at 2.5 μg/mL.
Application
Western blot analysis of OTUD5 in human kidney lysate with OTUD5 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | OTUD5 antibody can be used for detection of OTUD5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | OTUD5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | OTUD5 Antibody is supplied in PBS containing 0.02% sodium azide. |
