Application
Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | OTUD6B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OTU domain-containing protein 6B, DUBA-5, OTUD6B, DUBA5 |
| Product description | Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA5 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM].1) Kimura, K., et al. Genome Res. 16(1):55-65(2006) |
| Immunogen | This OTUD6B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-262 amino acids from the C-terminal region of human OTUD6B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OTUD6B |
|---|---|
| Alternative Names | OTU domain-containing protein 6B DUBA-5 OTUD6B DUBA5 |
| Molecular Weight(MW) | 34 kDa |
Application
Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
