Application
Western blot of Hela cell lysates that had been treated with +/- UV(30 min.) showing phosphospecific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182.| Product Name | p38 MAPK (phospho Thr180 / Tyr182) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RK, Hog, p38, CRK1, CSBP, Exip, Mxi2, CSPB1, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MAP kinase 14 |
| Product description | The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one these kinases and it is activated by both inflammatory cytokines and by stress. The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system. Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182.1) Shi, Y. and Gaestel, M. "In the cellular garden of forking paths: How p38 MAPKs signal for downstream assistance," Biol. Chem. 383 (2002) 1519 - 1536. |
| Immunogen | p38 MAPK (Thr180/Tyr182) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr180/Tyr182 of p38. |
| Modification | p-Thr180 / Tyr182 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Immunolabeling in Western blots of C-6 glioma cells prepared with anisomycin treatment is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Immunoblotting (DB) and Western Blots (WB). Immunohistochemistry (IHC): not yet determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Mapk14 |
|---|---|
| Alternative Names | RK Hog p38 CRK1 CSBP Exip Mxi2 CSPB1 Csbp1 Csbp2 Prkm14 Prkm15 Sapk2A p38Hog p38alpha MAP kinase 14 |
| Molecular Weight(MW) | 39 |
| Tissue Specificity | p38 MAPK antibody is specific for the ~39k p38 protein phosphorylated at Thr180/Tyr182. |
Application
Western blot of Hela cell lysates that had been treated with +/- UV(30 min.) showing phosphospecific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182.| Application Notes | Immunolabeling in Western blots of C-6 glioma cells prepared with anisomycin treatment is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Immunoblotting (DB) and Western Blots (WB). Immunohistochemistry (IHC): not yet determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
