Application
Immunocytochemistry of p53AIP1 in HL60 cells with p53AIP1 antibody at 10 μg/mL.Application
Immunofluorescence of p53AIP1 in HL60 cells with p53AIP1 antibody at 20 μg/mL.Application
Western blot analysis of p53AIP1 in HL60 cell lysate with p53AIP1 antibody at (A) 4 and (B) 8 μg/mL.| Product Name | p53AIP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p53AIP1 Antibody: P53AIP1, p53-regulated apoptosis-inducing protein 1, p53AIP1 |
| Product description | p53AIP1 Antibody: The p53 tumor-suppressor protein can induce apoptosis through transcriptional activation of several genes. One such protein p53AIP was initially identified through direct cloning of p53 binding sequences from human genomic DNA. Its expression is inducible by p53 following p53 phosphorylation on Ser-46, and ectopic expression of p53AIP leads to apoptotic cell death. Both the phosphorylation of p53 and the induction of p53AIP were blocked by inhibiting the expression of p53DINP1 by the introduction of antisense oligonucleotides to p53DINP1, suggesting that the apoptosis associated with p53AIP expression is regulated by p53DINP1. Finally, as adenovirus-mediated introduction of p53AIP has been shown to suppress tumor growth in vivo, it has been suggested that p53AIP gene transfer may become a useful strategy for the treatment of p53-resistant cancers. Three isoforms of p53AIP are known to exist; this antibody will detect all three.1) Kern SE, Pietenpol JA, Thiagalingam S, Seymour A, et al. Oncogenic forms of p53 inhibit p53-regulated gene expression. Science1991; 252:1708-11. |
| Immunogen | p53AIP1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human p53AIP1. The immunogen is located within the first 50 amino acids of p53AIP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| p53AIP1 antibody can be used for detection of p53AIP1 by Western blot at 4 - 8 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP53AIP1 |
|---|---|
| Alternative Names | p53AIP1 Antibody: P53AIP1 p53-regulated apoptosis-inducing protein 1 p53AIP1 |
| Sequence Similarities | 2) Cat. No. 17-009 - HL-60 Cell Slide |
Application
Immunocytochemistry of p53AIP1 in HL60 cells with p53AIP1 antibody at 10 μg/mL.
Application
Immunofluorescence of p53AIP1 in HL60 cells with p53AIP1 antibody at 20 μg/mL.
Application
Western blot analysis of p53AIP1 in HL60 cell lysate with p53AIP1 antibody at (A) 4 and (B) 8 μg/mL.| Positive Control | 1) Cat. No. 1209 - HL60 Cell Lysate |
|---|---|
| Application Notes | p53AIP1 antibody can be used for detection of p53AIP1 by Western blot at 4 - 8 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | p53AIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | p53AIP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
