Application
Immunohistochemical staining of mouse liver using p53DINP1 antibody at 2 μg/mL.Application
Western blot analysis of p53DINP1 expression in human lung tissue lysate with p53DINP1 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of p53DINP1 in human liver tissue with p53DINP1 antibody at 5 μg/ml.| Product Name | p53DINP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p53DINP1 Antibody: SIP, Teap, p53DINP1, TP53DINP1, TP53INP1A, TP53INP1B, P53DINP1, SIP, Tumor protein p53-inducible nuclear protein 1, Stress-induced protein |
| Product description | p53DINP1 Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible gene was identified recently and designated p53DINP1 (for p53-dependent damage-inducible nuclear protein 1) and SIP (for stress induced protein) in human and mouse. A p53DINP1 antisense oligonucleotide inhibits and overexpression of p53DINP1 enhances Ser46 phosphorylation of p53, induction of p53AIP1, and cell death induced by DNA double-strand breaks. p53DINP1 may regulate p53-dependent apoptosis through phosphorylation at Ser46 and induction of p53AIP1. The p53DINP1/SIP gene encodes two proteins of 27 and 18 kDa in human and mouse termed p53DINP1-alpha and p53DINP1-beta or SIP27 and SIP18. p53DINP1/SIP is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress.1) Okamura S, Arakawa H, Tanaka T, et al. p53DINP1, a p53-inducible gene, regulates p53-dependent apoptosis. Mol. Cell. 2001; 8:85-94. |
| Immunogen | p53DINP1 antibody was raised with a synthetic peptide corresponding to 14 amino acids near the amino terminus of human p53DINP1. The immunogen is located within the first 50 amino acids of p53DINP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| p53DINP1 antibody can be used for detection of p53DINP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP53INP1 |
|---|---|
| Alternative Names | p53DINP1 Antibody: SIP Teap p53DINP1 TP53DINP1 TP53INP1A TP53INP1B P53DINP1 SIP Tumor protein p53-inducible nuclear protein 1 Stress-induced protein |
| Molecular Weight(MW) | Predicted: 18, 27 kDa Observed: 30 kDa |
| Tissue Specificity | At least two isoforms of p53DINP1 are known to exist; this antibody will detect both isoforms. |
Application
Immunohistochemical staining of mouse liver using p53DINP1 antibody at 2 μg/mL.
Application
Western blot analysis of p53DINP1 expression in human lung tissue lysate with p53DINP1 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of p53DINP1 in human liver tissue with p53DINP1 antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | p53DINP1 antibody can be used for detection of p53DINP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | p53DINP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | p53DINP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
