Application
Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 μg/mL.Application
Immunohistochemistry of p53R2 in human lung tissue with p53R2 antibody at 1 μg/mL.Application
Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | p53R2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p53R2 Antibody: P53R2, MTDPS8A, MTDPS8B, P53R2, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53R2 |
| Product description | p53R2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and gamma-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression. p53R2 is an important target of p53 for tumor suppression.1) Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature 2000; 404:42-9. |
| Immunogen | p53R2 antibody was raised against a 16 amino acid peptide near the amino terminus of human p53R2. The immunogen is located within the first 50 amino acids of p53R2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| p53R2 antibody can be used for detection of p53R2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RRM2B |
|---|---|
| Alternative Names | p53R2 Antibody: P53R2 MTDPS8A MTDPS8B P53R2 Ribonucleoside-diphosphate reductase subunit M2 B TP53-inducible ribonucleotide reductase M2 B p53R2 |
| Molecular Weight(MW) | Predicted: 39 kDa Observed: 43 kDa |
| Sequence Similarities | 4) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | At least six isoforms of p53R2 are known to exist; this antibody will detect all but the largest isoform. |
Application
Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 μg/mL.
Application
Immunohistochemistry of p53R2 in human lung tissue with p53R2 antibody at 1 μg/mL.
Application
Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | p53R2 antibody can be used for detection of p53R2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | p53R2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | p53R2 Antibody is supplied in PBS containing 0.02% sodium azide. |
