Application
Western blot analysis of lysed extracts from K562, HL-60 and JK cells using p62Dok (Ab-398).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok (Ab-398). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p62Dok (Ab-398).| Product Name | p62Dok Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P62DOK, CK2A1, DOK1 |
| Product description | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.1) Michael J. Wick, et, al. (2001) J. Biol. Chem; 276: 42843 - 42850. Paul D. Simoncic, et, al. (2006) Mol. Cell. Biol; 26: 4149 - 4160. Nadia Dube, et, al. (2004) PNAS; 101: 1834. Adam C. Yopp, et al. (2005) J. Immunol; 175: 2913 - 2924. |
| Immunogen | p62Dok (Ab-398) antibody was raised against a peptide sequence around aa.396~400 (P-I-Y-D-E) derived from Human p62Dok. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DOK1 |
|---|---|
| Alternative Names | P62DOK CK2A1 DOK1 |
| Molecular Weight(MW) | 62 kDa |
| Tissue Specificity | This antibody detects endogenous level of total p62Dok protein. |
| Entrez Gene | 1796 |
|---|---|
| Protein Accession | NP_001372.1 |
Application
Western blot analysis of lysed extracts from K562, HL-60 and JK cells using p62Dok (Ab-398).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok (Ab-398). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p62Dok (Ab-398).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
