Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok (Phospho-Tyr398).Application
Western blot analysis of lysed extracts from K562 cells untreated or treated with H2O2 using p62Dok (phospho-Tyr398).| Product Name | p62Dok (phospho Tyr398) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P62DOK, ERBB, ERBB1, HER1, DOK1 |
| Product description | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.1) Michael J. Wick, et al. (2001) J. Biol. Chem ; 276: 42843 - 42850. Paul D. Simoncic, et al. (2006) Mol. Cell. Biol ; 26: 4149 - 4160. Nadia Dube, et al. (2004) PNAS ; 101: 1834. Adam C. Yopp, et al. (2005) J. Immunol ; 175: 2913 - 2924. |
| Immunogen | p62Dok (phospho-Tyr398) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 398 (E-G-Y (p) -E-L) derived from Human p62Dok. |
| Modification | p-Tyr398 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DOK1 |
|---|---|
| Alternative Names | P62DOK ERBB ERBB1 HER1 DOK1 |
| Molecular Weight(MW) | 62 kDa |
| Tissue Specificity | This antibody detects endogenous level of p62Dok only when phosphorylated at tyrosine 398. |
| Entrez Gene | 1796 |
|---|---|
| Protein Accession | NP_001372.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok (Phospho-Tyr398).
Application
Western blot analysis of lysed extracts from K562 cells untreated or treated with H2O2 using p62Dok (phospho-Tyr398).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
