Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of PABPN1L Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
PABPN1L Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:500-1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: human kidney lysate Lysates/proteins at 20 ug per lane.| Product Name | PABPN1L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Embryonic polyadenylate-binding protein 2, Embryonic poly(A)-binding protein 2, ePABP-2, ePABP2, Embryonic poly(A)-binding protein type II, Poly(A)-binding protein nuclear-like 1, PABPN1L, EPABP2, PABPNL1 |
| Product description | The function of this protein remains unknown. |
| Immunogen | This PABPN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of human PABPN1L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:500-1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PABPN1L |
|---|---|
| Alternative Names | Embryonic polyadenylate-binding protein 2 Embryonic poly(A)-binding protein 2 ePABP-2 ePABP2 Embryonic poly(A)-binding protein type II Poly(A)-binding protein nuclear-like 1 PABPN1L EPABP2 PABPNL1 |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Xenopus |
Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of PABPN1L Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
PABPN1L Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:500-1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: human kidney lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:500-1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
