Application
Western blot analysis of anti-PAD4 Pab in 293 cell line lysate| Product Name | PAD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein-arginine deiminase type-4, HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV, PADI4, PADI5, PDI5 |
| Product description | PAD4 is a member of a family of enzymes responsible for the conversion of arginine residues to citrulline residues via catalyzation of the deimination of the arginine residues. PAD4 down-regulates histone H3 and H4 arginine methylation, both by preventing arginine methylation by CARM1 and HRMT1L2/PRMT1 and by converting methylarginine to citrulline. This protein may play a role in granulocyte and macrophage development leading to inflammation and immune response.1) Nakayama-Hamada, M., et al., Biochem. Biophys. Res. Commun. 327(1):192-200 (2005). |
| Immunogen | This PAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 512-544 amino acids from the C-terminal region of human PAD4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PADI4 |
|---|---|
| Alternative Names | Protein-arginine deiminase type-4 HL-60 PAD Peptidylarginine deiminase IV Protein-arginine deiminase type IV PADI4 PADI5 PDI5 |
| Molecular Weight(MW) | 74 kDa |
Application
Western blot analysis of anti-PAD4 Pab in 293 cell line lysate| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
