| Product Name | PAG/Cbp Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphoprotein Associated with Glycosphingolipid-enriched Microdomains 1; Csk-binding Protein |
| Product description | PAG [Cbp] is a ubiquitously expressed transmembrane adapter protein. Depending on its phosphorylation status, it appears as diffuse band(s) of 80-90kDa by SDS-PAGE, with phosphorylation leading to a shift towards a higher apparent molecular mass. Tyrosine phosphorylated PAG recruits Csk to the membrane and negatively regulates Src family kinases via Csk-mediated phosphorylation. Upon T cell receptor engagement, PAG becomes dephosphorylated, thereby displacing Csk from the membrane and enabling the activation of the Src kinases Fyn and Lck. PAG negatively regulates TCR (T cell antigen receptor)-mediated signaling in T cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. It promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. It inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins and may be involved in cell adhesion signaling. |
| Immunogen | Synthetic peptide corresponding to aa 412-424 of the N-terminal rat PAG including an additional N-terminal Cys residue. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICCIPWB |
| Immunocytochemistry: (1:50). Immunoprecipitation: (5ug/sample) . Western Blot: (1:1,000) . Optimal conditions must be determined individually for each application.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Pag1 |
|---|---|
| Alternative Names | Phosphoprotein Associated with Glycosphingolipid-enriched Microdomains 1 Csk-binding Protein |
| Tissue Specificity | Recognizes mouse, rat and human PAG/Cbp. |
| Application Notes | Immunocytochemistry: (1:50). Immunoprecipitation: (5ug/sample) . Western Blot: (1:1,000) . Optimal conditions must be determined individually for each application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. In PBS containing 0.02% sodium azide. |
