Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).Application
PAICS Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | PAICS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Multifunctional protein ADE2, Phosphoribosylaminoimidazole-succinocarboxamide synthase, SAICAR synthetase, Phosphoribosylaminoimidazole carboxylase, AIR carboxylase, AIRC, PAICS, ADE2, AIRC, PAIS |
| Product description | This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene.1) Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010) |
| Immunogen | This PAICS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-123 amino acids from the N-terminal region of human PAICS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAICS |
|---|---|
| Alternative Names | Multifunctional protein ADE2 Phosphoribosylaminoimidazole-succinocarboxamide synthase SAICAR synthetase Phosphoribosylaminoimidazole carboxylase AIR carboxylase AIRC PAICS ADE2 AIRC PAIS |
| Molecular Weight(MW) | 47 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Application
PAICS Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
