Application
Immunohistochemistry of PAK4 in human colon tissue with PAK4 antibody at 10 μg/mL.Application
Western blot analysis of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of PAK4 in Human Colon tissue with PAK4 antibody at 20 μg/mL.| Product Name | PAK4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAK4 Antibody: KIAA1142, p21-activated kinase 4, PAK-4 |
| Product description | PAK4 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.1) Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7. |
| Immunogen | PAK4 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4. The immunogen is located within amino acids 210 - 260 of PAK4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PAK4 antibody can be used for the detection of PAK4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK4 |
|---|---|
| Alternative Names | PAK4 Antibody: KIAA1142 p21-activated kinase 4 PAK-4 |
| Molecular Weight(MW) | Predicted: 65 kDa Observed: 70 kDa |
| Sequence Similarities | 2) Cat. No. 1320 - Human Colon Tissue Lysate |
| Tissue Specificity | PAK4 antibody is predicted to not cross-react with other PAK family proteins. |
Application
Immunohistochemistry of PAK4 in human colon tissue with PAK4 antibody at 10 μg/mL.
Application
Western blot analysis of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of PAK4 in Human Colon tissue with PAK4 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1221 - SW480 Cell Lysate |
|---|---|
| Application Notes | PAK4 antibody can be used for the detection of PAK4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PAK4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PAK4 Antibody is supplied in PBS containing 0.02% sodium azide. |
