WB
Western Blot:Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not).All lanes Phospho-PAK4/PAK5/PAK6 antibody at 2.15μg/ml,Secondary:Goat polyclonal to rabbit IgG at 1/50000 dilution.ICC/IF
Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).| Product Name | Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from Human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) |
| Modification | p-S474/S560/S602 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:5000 ICC/IF:1:20-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK4 |
|---|---|
| Gene Full Name | p21 (RAC1) activated kinase 4 |
| Gene Summary | PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 64,75,80 kDa(Observed band size: 75,80 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western Blot:Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not).All lanes Phospho-PAK4/PAK5/PAK6 antibody at 2.15μg/ml,Secondary:Goat polyclonal to rabbit IgG at 1/50000 dilution.
ICC/IF
Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).| Application Notes | WB:1:500-1:5000 ICC/IF:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
