Application
Immunocytochemistry of PAK5 in K562 cells with PAK5 antibody at 2 μg/mL.Application
Immunofluorescence of PAK5 in K562 cells with PAK5 antibody at 10 μg/mL.Application
Western blot analysis of PAK5 in T24 lysate with PAK5 antibody at (A) 2 and (B) 4 μg/mL.| Product Name | PAK5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAK5 Antibody: PAK5, KIAA1264, PAK5, p21-activated kinase 5, PAK-5 |
| Product description | PAK5 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK5 was initially identified as a kinase expressed primarily in brain that while possessing a kinase domain and GTPase binding domain similar to PAK4 and PAK6, is completely different from both. Expression of PAK5 in neural based cell lines resulted in neurite outgrowth suggesting that PAK5 may be involved in regulating the cytoskeletal changes necessary for promoting neurite outgrowth. Other experiments suggest that unlike the other PAKs, PAK5 may inhibit apoptosis by phosphorylating the Bcl-2 family member Bad.1) Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7. |
| Immunogen | PAK5 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK5. The immunogen is located within amino acids 240 - 290 of PAK5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PAK5 antibody can be used for the detection of PAK5 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK7 |
|---|---|
| Alternative Names | PAK5 Antibody: PAK5 KIAA1264 PAK5 p21-activated kinase 5 PAK-5 |
| Sequence Similarities | 3) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunocytochemistry of PAK5 in K562 cells with PAK5 antibody at 2 μg/mL.
Application
Immunofluorescence of PAK5 in K562 cells with PAK5 antibody at 10 μg/mL.
Application
Western blot analysis of PAK5 in T24 lysate with PAK5 antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1213 - T24 Cell Lysate |
|---|---|
| Application Notes | PAK5 antibody can be used for the detection of PAK5 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PAK5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PAK5 Antibody is supplied in PBS containing 0.02% sodium azide. |
