Application
Western blot analysis of extracts from HeLa cells (Lane 2), using PAK5/6 (Phospho-Ser602/Ser560) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | PAK5/6 (phospho Ser602/Ser560) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAK5, PAK 6, kinase PAK6 |
| Product description | This gene encodes a protein that shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development.1) Yang F., J. Biol. Chem. 276:15345-15353(2001). |
| Immunogen | PAK5/6 (phospho Ser602/Ser560) antibody was raised against a peptide sequence around phosphorylation site of Serine 602/560 (R-K-S (p)-L-V) derived from Human PAK5/6. |
| Modification | p-Ser602/Ser560 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK5, PAK6 |
|---|---|
| Alternative Names | PAK5 PAK 6 kinase PAK6 |
| Molecular Weight(MW) | 75 kDa |
| Tissue Specificity | The antibody detects endogenous levels of PAK5/6 only when phosphorylated at serine 602/560. |
| Entrez Gene | 57144/56924 |
|---|---|
| Protein Accession | NP_065074.1 |
Application
Western blot analysis of extracts from HeLa cells (Lane 2), using PAK5/6 (Phospho-Ser602/Ser560) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
