Application
Immunofluorescence of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.Application
Immunocytochemistry of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.Application
Western blot analysis of PAK6 in Raji lysate with PAK6 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.| Product Name | PAK6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAK6 Antibody: PAK5, PAK5, PAK-5, PAK-6 |
| Product description | PAK6 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.1) Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7. |
| Immunogen | PAK6 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK6. The immunogen is located within amino acids 260 - 310 of PAK6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PAK6 antibody can be used for the detection of PAK6 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK6 |
|---|---|
| Alternative Names | PAK6 Antibody: PAK5 PAK5 PAK-5 PAK-6 |
| Molecular Weight(MW) | Predicted: 75 kDa Observed: 70 kDa |
| Sequence Similarities | 2) Cat. No. 17-007 - Raji Cell Slide |
| Tissue Specificity | PAK6 antibody is predicted to not cross-react with other PAK family proteins. |
Application
Immunofluorescence of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.
Application
Immunocytochemistry of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.
Application
Western blot analysis of PAK6 in Raji lysate with PAK6 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | PAK6 antibody can be used for the detection of PAK6 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PAK6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PAK6 Antibody is supplied in PBS containing 0.02% sodium azide. |
