Application
Western blot analysis of PALB2 in SK-N-SH cell lysate with PALB2 antibody at 1 μg/mL.| Product Name | PALB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PALB2 Antibody: The breast cancer early onset proteins BRCA1 and BRCA2 are central to the repair of DNA damage by homologous recombination and are strongly associated with inherited breast and ovarian cancer. The protein PALB2 is required for the localization of BRCA2 to sites of DNA damage and like BRCA1 and 2 is a breast cancer susceptibility gene. PALB2 is thought to function through directly binding to BRCA1, which allows the PALB2 protein to organize BRCA2 and the recombinase RAD51 at the site of DNA damage. Analysis of the BRCA1-PALB2-BRCA2-RAD51network may allow predictions to be made of the responsiveness of a particular tumor to therapeutic treatments.1) Greenberg RA. Recognition of DNA double strand breaks by the BRCA1 tumor suppressor network. Chromosoma 2008; 117:305-17. |
| Immunogen | PALB2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human PALB2. The immunogen is located within amino acids 940 - 990 of PALB2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PALB2 antibody can be used for detection of PALB2 by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PALB2 |
|---|---|
| Alternative Names | PALB2 Antibody: FANCN PNCA3 FANCN Partner and localizer of BRCA2 |
| Molecular Weight(MW) | Predicted: 65, 100, 130 kDa Observed: 140 kDa |
| Tissue Specificity | At least four isoforms of PALB2 are known to exist; this antibody will detect all four isoforms. |
Application
Western blot analysis of PALB2 in SK-N-SH cell lysate with PALB2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | PALB2 antibody can be used for detection of PALB2 by Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | PALB2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PALB2 Antibody is supplied in PBS containing 0.02% sodium azide. |
