Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
PAN3 Antibody IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of PAN3 Antibody in Jurkat cell line lysates (35ug/lane)| Product Name | PAN3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PAN3 is functions in cytoplasmic mRNA decay. It is as part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity.1) Uchida, N., et al. J. Biol. Chem. 279(2):1383-1391(2004) |
| Immunogen | This PAN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 771-799 amino acids from the C-terminal region of human PAN3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAN3 |
|---|---|
| Alternative Names | PAB-dependent poly(A)-specific ribonuclease subunit PAN3 {ECO:0000255|HAMAP-Rule:MF_03181} PAB1P-dependent poly(A)-nuclease {ECO:0000255|HAMAP-Rule:MF_03181} PAN deadenylation complex subunit 3 {ECO:0000255|HAMAP-Rule:MF_03181} hPan3 PAN3 {ECO:0000255| |
| Molecular Weight(MW) | 96 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
PAN3 Antibody IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of PAN3 Antibody in Jurkat cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
