Application
PAP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | PAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Poly(A) polymerase alpha, PAP-alpha, Polynucleotide adenylyltransferase alpha, PAPOLA, PAP |
| Product description | Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.1) Rapti, A., et al. Mol. Cell. Biochem. 340 (1-2), 91-96 (2010) : |
| Immunogen | This PAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-435 amino acids from the Central region of human PAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAPOLA |
|---|---|
| Alternative Names | Poly(A) polymerase alpha PAP-alpha Polynucleotide adenylyltransferase alpha PAPOLA PAP |
| Molecular Weight(MW) | 83 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
PAP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
