Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | PAPOLG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAPOLG, FLJ11805, FLJ12972, FLJ13482, FLJ14187, MGC133307, MGC133308, PAP2, PAPG |
| Product description | PAPOLG is a member of the poly (A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly (A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity.This gene encodes a member of the poly (A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly (A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14.1) Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAPOLG. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PAPOLG antibody can be used for detection of PAPOLG by ELISA at 1:1562500. PAPOLG antibody can be used for detection of PAPOLG by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAPOLG |
|---|---|
| Alternative Names | PAPOLG FLJ11805 FLJ12972 FLJ13482 FLJ14187 MGC133307 MGC133308 PAP2 PAPG |
| Molecular Weight(MW) | 83 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | PAPOLG antibody can be used for detection of PAPOLG by ELISA at 1:1562500. PAPOLG antibody can be used for detection of PAPOLG by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAPOLG antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
