This gene encodes a member of the poly(A) polymerase
family which catalyzes template-independent extension of the 3' end
of a DNA/RNA strand. This enzyme shares 60% identity to the well
characterized poly(A) polymerase II (PAPII) at the amino acid
level. These two enzymes have similar organization of structural
and functional domains. This enzyme is exclusively localized in the
nucleus and exhibits both nonspecific and CPSF (cleavage and
polyadenylation specificity factor)/AAUAAA-dependent
polyadenylation activity. This gene is located on chromosome 2 in
contrast to the PAPII gene, which is located on chromosome 14.1) Lee, Y.S., et al. J. Biol. Chem. 285(36):28105-28116(2010)
Immunogen
This PAPOLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 685-714 amino acids from the C-terminal region of human PAPOLG.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.