Application
Immunocytochemistry of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 5 μg/mL.Application
Immunofluorescence of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 20 μg/mL.Application
Western blot analysis of Paralemmin3 in MCF7 cell lysate with Paralemmin3 antibody at 1 μg/mL.| Product Name | Paralemmin3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Paralemmin3 Antibody: Paralemmin-3 |
| Product description | Paralemmin3 Antibody: Paralemmin3 is a recently identified protein that associates with the single immunoglobulin IL-1 receptor-related molecule (SIGIRR), a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Silencing of Paralemmin3 expression by RNAi inhibited the release of inflammatory cytokines in A549 cells after stimulation with lipopolysaccharide (LPS), suggesting Paralemmin3 may function as an adaptor in the LPS-TLR4 signaling pathway.1) Chen X, Wu X, Zhao Y, et al. A novel binding protein of single immunoglobulin IL-1 receptor-related molecule: Paralemmin-3. Biochem. Biophys. Res. Commun. 2011; 404:1029-33 |
| Immunogen | Paralemmin3 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human Paralemmin3. The immunogen is located within amino acids 50 - 100 of Paralemmin3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Paralemmin3 antibody can be used for detection of Paralemmin3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PALM3 |
|---|---|
| Alternative Names | Paralemmin3 Antibody: Paralemmin-3 |
| Sequence Similarities | 2) Cat. No. 17-012 - MCF7 Cell Slide |
| Tissue Specificity | At least two isoforms of Paralemmin3 are known to exist; this antibody will only detect the longer isoform |
| Entrez Gene | 342979 |
|---|---|
| Protein Accession | NP_001138500 |
Application
Immunocytochemistry of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 5 μg/mL.
Application
Immunofluorescence of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 20 μg/mL.
Application
Western blot analysis of Paralemmin3 in MCF7 cell lysate with Paralemmin3 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | Paralemmin3 antibody can be used for detection of Paralemmin3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Paralemmin3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Paralemmin3 Antibody is supplied in PBS containing 0.02% sodium azide. |
