Application
Immunocytochemistry of Parc in Daudi cells with Parc antibody at 1 μg/mL.Application
Immunofluorescence of Bcl-G in Daudi cells with Bcl-G antibody at 2 μg/mL.Application
Western blot analysis of PARC in Daudi lysate with PARC antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PARC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARC Antibody: PARC, H7AP1, KIAA0708, PARC, Cullin-9, UbcH7-associated protein 1, CUL-9 |
| Product description | PARC Antibody: The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase has recently been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression of this protein promoted cytoplasmic sequestration of p53. Surprisingly, PARC knockout mice were viable and exhibited no obvious phenotype, suggesting that other proteins, such as the highly related cullin family of E3 ubiquitin ligases, may perform similar functions in the absence of PARC. Additionally, it has been suggested that p53 binding to PARC may serve to control PARC function.1) Nikolaev AY, Li M, Puskas N, et al. Parc: a cytoplasmic anchor for p53. Cell 2003; 112:29-40. |
| Immunogen | PARC antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PARC. The immunogen is located within the last 50 amino acids of PARC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PARC antibody can be used for the detection of PARC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CUL9 |
|---|---|
| Alternative Names | PARC Antibody: PARC H7AP1 KIAA0708 PARC Cullin-9 UbcH7-associated protein 1 CUL-9 |
Application
Immunocytochemistry of Parc in Daudi cells with Parc antibody at 1 μg/mL.
Application
Immunofluorescence of Bcl-G in Daudi cells with Bcl-G antibody at 2 μg/mL.
Application
Western blot analysis of PARC in Daudi lysate with PARC antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | PARC antibody can be used for the detection of PARC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PARC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PARC Antibody is supplied in PBS containing 0.02% sodium azide. |
