Application
Immunohistochemistry of PARK2 in human brain tissue with PARK2 antibody at 5 μg/ml.Application
Immunofluorescence of PARK2 in human brain tissue with PARK2 antibody at 20 μg/ml.Application
Western blot analysis of PARK2 in human cerebellum tissue lysate with PARK2 antibody at 1 μg/ml.| Product Name | PARK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARK2 Antibody: PDJ, PRKN, AR-JP, LPRS2, E3 ubiquitin-protein ligase parkin, Parkinson juvenile disease protein 2, Parkinson disease protein 2 |
| Product description | Parkinson’s disease (PD) is a neurodegenerative disease whose symptoms include tremors, rigidity, bradykinesia, and postural instability (1). Mutations in the PARK2 gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease (2). The PARK2 protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation (3). Recent studies have suggested that PARK2 expression reduces the mitochondrial accumulation of the apoptosis protein Bax under basal conditions and directly ubiquitinates Bax, thereby promoting cell survival (4).1) Dauer W and Przedborski S. Parkinson’s disease: Mechanisms and models. Neuron 2003; 39:889-909. |
| Immunogen | PARK2 antibody was raised against a 17 amino acid peptide near the amino terminus of human PARK2. The immunogen is located within amino acids 110 - 160 of PARK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PARK2 antibody can be used for detection of PARK2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PARK2 |
|---|---|
| Alternative Names | PARK2 Antibody: PDJ PRKN AR-JP LPRS2 E3 ubiquitin-protein ligase parkin Parkinson juvenile disease protein 2 Parkinson disease protein 2 |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 55 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | PARK2 antibody is human specific. |
Application
Immunohistochemistry of PARK2 in human brain tissue with PARK2 antibody at 5 μg/ml.
Application
Immunofluorescence of PARK2 in human brain tissue with PARK2 antibody at 20 μg/ml.
Application
Western blot analysis of PARK2 in human cerebellum tissue lysate with PARK2 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1362 - Human Cerebellum Tissue Lysate |
|---|---|
| Application Notes | PARK2 antibody can be used for detection of PARK2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PARK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PARK2 antibody is supplied in PBS containing 0.02% sodium azide. |
