Application
Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S101 mutant.| Product Name | Parkin (phospho Ser101) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDJ, PRKN, AR-JP, LPRS2, Parkinson juvenile disease protein 2, Parkinson disease protein 2 |
| Product description | Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner aet al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).1) Jenner P, Dexter DT, Sian J, Schapira AH, Marsden CD (1992) Oxidative stress as a cause of nigral cell death in Parkinson’s disease and incidental Lewy body disease. Ann Neurol. 32 Suppl: S82-7. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser101 of human parkin. |
| Modification | p-Ser101 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with bovine and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|
| Species Reactivity | BovineHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PARK2 |
|---|---|
| Alternative Names | PDJ PRKN AR-JP LPRS2 Parkinson juvenile disease protein 2 Parkinson disease protein 2 |
| Molecular Weight(MW) | 52 |
Application
Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S101 mutant.| Application Notes | The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with bovine and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Parkin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
