Application
PARK8 (LRRK2) Antibody (E519)detect over-expressed human LRRK2 protein.Application
Fresh frozen tissue samples from the human temporal lobe (Htl) is subjected to different extraction buffers(Htl/Low Salt, Htl/high salt, Htl/1%triton, Htl/RIPA, Htl/1%SDS, HEK293T-pcDNA3, HEK293T-hLRRK2). PARK8 (LRRK2) Antibody (E519)has sufficient specificity to detect endogenous human LRRK2.| Product Name | PARK8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin, LRRK2, PARK8 |
| Product description | Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson's disease identified to date. LRRK2, a genetic mutation, was recently found linked to about 5 percent of inherited cases of Parkinson's disease. By high-resolution recombination mapping and candidate gene sequencing in 46 families, 6 disease-segregating mutations (5 missense and 1 putative splice site mutation). It may be central to the pathogenesis of several major neurodegenerative disorders associated with parkinsonism. LRRK2 belongs to the ROCO protein family and includes a protein kinase domain of the MAPKKK class and several other major functional domains.1) Shen J. Neuron. 2004. 44:575. |
| Immunogen | This PARK8(LRRK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 504-536 amino acids from human PARK8(LRRK2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | LRRK2 |
|---|---|
| Alternative Names | Leucine-rich repeat serine/threonine-protein kinase 2 Dardarin LRRK2 PARK8 |
| Molecular Weight(MW) | 286 kDa |
Application
PARK8 (LRRK2) Antibody (E519)detect over-expressed human LRRK2 protein.
Application
Fresh frozen tissue samples from the human temporal lobe (Htl) is subjected to different extraction buffers(Htl/Low Salt, Htl/high salt, Htl/1%triton, Htl/RIPA, Htl/1%SDS, HEK293T-pcDNA3, HEK293T-hLRRK2). PARK8 (LRRK2) Antibody (E519)has sufficient specificity to detect endogenous human LRRK2.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
