PARP2 contains a catalytic domain and is capable of catalyzing a poly (ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of protein.1) Malanga, M. et al., (2004) J. Biol. Chem. 279 (7), 5244-5248.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PARP2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PARP2 antibody can be used for detection of PARP2 by ELISA at 1:62500. PARP2 antibody can be used for detection of PARP2 by western blot at 1.6 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IF on Rat thyrocytes-FRTL-5 at 1:100.
Application
Antibody used in WB on Human Raji cells at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1207 - Raji Cell Lysate
Application Notes
PARP2 antibody can be used for detection of PARP2 by ELISA at 1:62500. PARP2 antibody can be used for detection of PARP2 by western blot at 1.6 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PARP2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.