Application
PARS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PARS2 using rabbit polyclonal PARS2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PARS2 gene.Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).| Product Name | PARS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable proline--tRNA ligase, mitochondrial, Prolyl-tRNA synthetase, ProRS, PARS2 |
| Product description | Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.1) Lamesch, P., et al. Genomics 89(3):307-315(2007) |
| Immunogen | This PARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-417 amino acids from the C-terminal region of human PARS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PARS2 |
|---|---|
| Alternative Names | Probable proline--tRNA ligase mitochondrial Prolyl-tRNA synthetase ProRS PARS2 |
| Molecular Weight(MW) | 53 kDa |
Application
PARS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PARS2 using rabbit polyclonal PARS2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PARS2 gene.
Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
