The tumor suppressor WT1 represses and activates transcription. Anti-Prostate Apoptosis Response Protein Par-4 (PAWR) is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.1) Bergmann, M., et al., (2004) Ann.Hematol.83(10), 646-653.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAWR.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
PAWR antibody can be used for detection of PAWR by ELISA at 1:62500. PAWR antibody can be used for detection of PAWR by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Jurkat 0.5-1.0 ug/ml.
Application
Antibody used in IHC on Human Liver.
Application
Antibody used in IHC on Human kidney.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
PAWR antibody can be used for detection of PAWR by ELISA at 1:62500. PAWR antibody can be used for detection of PAWR by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAWR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 200 μL of distilled water. Final antibody concentration is 1 mg/mL.