The PAX genes, including PAX1, are a highly conserved family of developmental control genes that encode transcription factors and have been shown to play a role in pattern formation during embryogenesis in vertebrates.The PAX genes, including PAX1, are a highly conserved family of developmental control genes that encode transcription factors and have been shown to play a role in pattern formation during embryogenesis in vertebrates (McGaughran et al., 2003 [PubMed 12774041]). See PAX7 (MIM 167410) for a discussion of paired box domain genes.1) Vatanavicharn, N., (2007) Am. J. Med. Genet. A 143 (19), 2292-2302.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAX1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PAX1 antibody can be used for detection of PAX1 by ELISA at 1:312500. PAX1 antibody can be used for detection of PAX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
PAX1 antibody can be used for detection of PAX1 by ELISA at 1:312500. PAX1 antibody can be used for detection of PAX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.