Application
Western blot analysis in HepG2,MDA-MB453,293,K562,MCF-7 cell line lysates (35ug/lane).Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PAX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The PAX genes, including PAX1, are a highly conserved family of developmental control genes that encode transcription factors and have been shown to play a role in pattern formation during embryogenesis in vertebrates (McGaughran et al., 2003 [PubMed 12774041]). See PAX7 (MIM 167410) for a discussion of paired box domain genes.1) Huang, T.H., et al. Int. J. Gynecol. Cancer 20(4):513-519(2010) |
| Immunogen | This PAX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-471 amino acids from the C-terminal region of human PAX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAX1 |
|---|---|
| Alternative Names | Paired box protein Pax-1 HuP48 PAX1 HUP48 |
| Molecular Weight(MW) | 55 kDa |
Application
Western blot analysis in HepG2,MDA-MB453,293,K562,MCF-7 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For FACS starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
